Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers

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Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers

BACKGROUND It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers. METHODS In experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the z...

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The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers

OBJECTIVE We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. METHODS Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona opening performed at the site of the ICM, n=125), the trophectoderm (TE) group (zona opening performed opposite to the ICM, n=125) a...

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The effects of laser assisted hatching on pregnancy rates

BACKGROUND For infertile women aged over 35 years, failure of the ZP (zona pellucida) to rupture is believed to be associated with a decreased implantation rate in in vitro fertilization (IVF) or intra cytoplasmic sperm injection (ICSI). OBJECTIVE In this research, laser assisted hatching (LAH) was offered to patients with advanced maternal age to evaluate a possible benefit. MATERIALS AND ...

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the effects of laser assisted hatching on pregnancy rates

abstract background: for infertile women aged over 35 years, failure of the zp (zona pellucida) to rupture is believed to be associated with a decreased implantation rate in in vitro fertilization (ivf) or intra cytoplasmic sperm injection (icsi). objective: in this research, laser assisted hatching (lah) was offered to patients with advanced maternal age to evaluate a possible benefit. materia...

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effect of l-carnitine on in vitro developmental rate, the zona pellucida and hatching of blastocysts and their cell numbers in mouse embryos

abstractl-carnitine (lc) is an antioxidant with the ability to promote the growth in vitro embryo.objective: the goal was to evaluate the effect of lc on some indicators of embryo development and blastocyst quality including zona pellucid (zp) thickness, the hatching of blastocysts and their cell numbers.materials and methods: mouse embryos were randomly divided into five groups and incubated w...

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ژورنال

عنوان ژورنال: Reproductive Biology and Endocrinology

سال: 2013

ISSN: 1477-7827

DOI: 10.1186/1477-7827-11-21